VIB-VUB Center for Structural Biology Tompa Lab

Research

RESEARCH PROJECTS (2022-2024)

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Biomolecular condensates of intrinsically disordered proteins

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Application of PSS for inhibiting toxicity of ALS/FTD-linked DPRs C9orf72-linked R-DPRs aggravate pathological phase separation of G3BP1 Phase-separating fusion proteins drive cancer by dysregulating transcription Biological colloids: Unique properties of membraneless organelles in the cell F/YGG-motif is an intrinsically disordered nucleic-acid binding motif The role of ordered cooperative assembly in biomolecular condensates LLPS affects self-aggregation and seeded aggregation of TDP-43 LCD Studying the kinetics of LLPS under near-native conditions LLPS of C9orf72 dipeptide repeats perturbs stress granule dynamics

Macromolecular interactions and liquid-liquid phase separation of the human androgen receptor

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Menin regulates AR/MLL-driven condensation and modulates transcription programs RNA-dependent LLPS driven by AR-DBD The effect of mono-amino acid repeats on the function of AR

Proteomics systems biology data integration to contrast the healthy and pathogenic cellular regulation of pathways

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Phase-separating fusion proteins drive cancer by dysregulating transcription Degron masking outlines degronons, co-degrading modules in the proteome Data integration highlights concentration & dosage sensitivity of LLPS drivers

Macromolecular modeling and systematic analysis of IDPs & IDP complexes

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IDPs uncouple affinity from binding specificity Challenges in describing the dynamics of proteins with ambiguous behavior PED 4.0: a DB for the conformational ensembles of IDPs Coevolutionary signals in IDP complexes Distance-based metrics for comparing IDP ensembles Interaction between the scaffold proteins CBP by IQGAP1 IDPs enable functional diversity while minimizing promiscuity CBP-ID3 transiently interacts with an IDR of ZFP106 while remaining 'fuzzy'
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Harnessing frog and gecko glues for biotechnological applications

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Giorgio Russo's vision FWO-SB: Unravelling the molecular mechanism of adhesive secretions for bioinspi…

COMPLETED RESEARCH PROJECTS

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Novel purification strategies for IDPs

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Novel Tandem-Tag Purification Strategy for Challenging IDPs Calpain purification through calpastatin and calcium: Strategy & procedures

Structure-based modulation of the calpastatin-calpain interaction

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Targeting calpastatin-like IDPs by covalent modifications Calpain purification through calpastatin and calcium: Strategy & procedures In vivo biotinylated calpastatin improves the affinity purification of calpain

Regulated protein degradation in the ubiquitin-proteasome system

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Degron masking outlines degronons, co-degrading modules in the proteome Design principles facilitating substrate selection and degradation by the UPS Tripartite degrons confer specificity on regulated protein degradation Functional diversity and structural disorder in the UPS pathway

Structural & functional study on ERD (Early Response to Dehydration) plant proteins

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Disordered short binding elements in the chaperone function of ERD14 ERD14 activates GSTF9 under osmotic stress 1H, 15N, 13C-NMR resonance assignment of the plant ERD10 protein